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BACKGROUND Patch testing is the criterion standard for diagnosis of allergic contact dermatitis (ACD). OBJECTIVE The aim of the study was to report the trends of patch testing results with the standard series at Massachusetts General Hospital from January 1, 2007, to December 31, 2016, compared with previous data from 1998 to 2006 and from 1990 to 2006 and those reported by the North American Contact Dermatitis Group. METHODS Data were collected and analyzed from retrospective chart reviews, focusing on 50 allergens in our standard ser