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Further, the morphological analysis showed the significant changes in TiO2 nanoparticles treated cells and the nanocomposite induces the myotube formation due to the increased ROS level in bovine satellite cells. Moreover, the nanocomposite regulates the expression of genes IGF-1, TGF-β, MSTN, CASP3, CASP2 and proteins such as CALP1, CALP2, MyoD, MyoG which are responsible for the growth, proliferation, and differentiation of satellite cells. Together, the prepared Ti@Fe2O3 nanocomposites afford additional support for the applications of