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Objectives To explore the prevalence of obstructive sleep apnea (OSA) within a professional rugby league team and determine associations of OSA with ethnicity, positional group, and physical characteristics. Design Observational prospective cohort study. Methods Twenty-two professional rugby league athletes underwent one night of home-based polysomnography with apnea-hypopnea index (AHI), rapid eye movement (REM)AHI, non-REMAHI and supineAHI determined to indicate OSA. Linear models were used to assess if playing position (back or forwar