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Thyroid cancer (TC) is a common malignant tumor, however the role of total vitamin D 25(OH)D, Platelet Derived Growth Factor (PDGF) and Insulin Like Growth Factor 1 (IGF-1) in the development of TC is still unclear. To assess the roles of 25(OH)D, PDGF and IGF-1 in the progression of thyroid diseases. The serum levels of 25(OH)D, PDGF and IGF-1 were assessed in 70 patients with papillary thyroid cancer (PTC), 60 patients with benign thyroid nodules (BN) compared to 60 normal controls (NC) using ELISA technique. There was a significant de