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© 2020 The Author(s). Published by Portland Press restricted on the behalf of the Biochemical Society.Axl, an associate for the TAM (Tyro3, AXL, Mer) receptor tyrosine kinase family members, plays critical functions in cell development, expansion, apoptosis, and migration. In this study, we demonstrated that the anti-cancer task of bufalin, an important bioactive element of the Chinese traditional medication Chan Su, is mediated by the downregulation of Axl in non-small-cell lung cancer (NSCLC) cells.