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The interest for graphene-based nanomaterials (GBMs) is growing worldwide as their properties allow the development of new innovative applications. In parallel, concerns are increasing about their potential adverse effects on the environment are increasing. The available data concerning the potential risk associated to exposure of aquatic organisms to these GBMs are still limited and little is known regarding their endocrine disruption potential. In the present study, the endocrine disruption potential of graphene oxide (GO) and reduced