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Methods aimed at boosting β-cell regeneration have long been pursued, but methods for reliably inducing individual β-cell expansion with complete retention of crucial features such glucose-stimulated insulin secretion (GSIS) remain not a lot of. We now have formerly reported that overexpression of this homeobox transcription element NKX6.1 promotes β-cell proliferation, while also enhancing GSIS and providing protection against β-cell cytotoxicity through induction for the VGF prohormone. We develop