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To research various other genotypes circulating in Tanzania and Ghana, we performed molecular genotyping on isolates from Tanzania and Ghana utilizing the MinION, a third-generation portable sequencing device from Oxford Nanopore Technologies. Utilising the MinION, we effectively sequenced the NDV F gene hypervariable region of 24 isolates from Tanzania and four samples from Ghana. In Tanzania, genotypes V, VII and XIII were recognized. All isolates from Ghana belonged to genotype XVIII. The