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For longitudinal studies, we additionally advise to set up an expert panel with a fixed number of assessors performing each evaluation in duplicate.(1) Background To evaluate the characteristics of fluorescein breakup patterns (FBUPs) among patients with dry eye disease (DED) and efficacy of FBUPs as a diagnostic test for DED subgroups. (2) Methods The study enrolled 449 patients with DED. FBUPs were categorized as follows area break (A, line break (L, spot break (S, dimple break (D, and random break (R. Schirmer value, fluores
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