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Here, we report the cryo-EM structure associated with personal b0,+AT-rBAT complex alone plus in complex with arginine substrate at resolution of 2.7 and 2.3 Å, correspondingly. The general structure of b0,+AT-rBAT is present as a dimer of heterodimer consistent with the previous study. A ligand molecule is likely to the substrate binding pocket, near which an occluded pocket is identified, to which we discovered that it's important for substrate transport.Shear thickening corresponds to an increase of the v