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These conclusions highlight significant region-specific differences in muscle dimensions and high quality among more youthful and older men and women, and emphasize the necessity to correct for subcutaneous fat depth when examining EI. Novelty Bullets • Rectus femoris CSA is comparable between younger and older adults except at most proximal web site evaluated. • Age- and sex-specific variations in uncorrected EI tend to be non-uniform throughout the thigh. • modification for subcutaneous fat width consid