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The element oxygen is expected to be a low-cost, strengthening element of titanium alloys due to its strong solid solution strengthening effect. High cycle fatigue behaviors of Ti-6Al-4V alloys with different oxygen contents (0.17%, 0.20%, 0.23% wt.%) were investigated in this paper. The results illustrated that Ti-6Al-4V-0.20O alloy possesses the highest fatigue strength and the lowest fatigue crack propagation rate. The fatigue fracture morphology verified that the fatigue cracks propagated transgranularly in both Ti-6Al-4V-0.17O and T