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The coupling between constructed wetlands and reservoir (CWs-R) afforded a novel ecosystem to improve the water quality and increase the emergency storage capacity of micro-polluted river drinking water source. In this study, spectroscopic characteristics of DOM in YL CWs-R ecosystem were first systematic studied based on a three-year field monitoring to investigate the chemical composition, sources and track the involved biogeochemical processes in the ecosystem. Three humic-like components (C1, C2, and C4, em 380 nm) and one prot