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OBJECTIVE To assess the performance of entire genome sequencing (WGS) for predicting Mycobacterium tuberculosis (MT medication opposition. TECHNIQUES 276 rifampin-resistance tuberculosis (RR-T and 30 rifampicin-sensitive clinical isolates had been arbitrarily chosen from patients with tuberculosis in Shanghai Pulmonary Hospital (SPH). Phenotypic drug susceptibility evaluation (DST) against 6 anti-TB medications were done, and WGS ended up being carried out to predict the medici