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Temperature could influence protein and amino acid deposition as well as gut microbiota profile and composition. However, the specific effects of ambient temperature on amino acids deposition and gut microbiota composition remain insufficiently understood. 300 one-day-old Avian broilers were randomly divided into three groups and reared at high, medium and low temperature (HF, MF and LF), respectively. Breast muscle and fecal samples were collected for amino acid composition analysis and 16S rRNA gene sequence analysis. Our data showed
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