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Published by Oxford University Press on behalf of Nucleic Acids Research.Multi-copy ampliconic gene families on the Y chromosome play an important role in spermatogenesis. Thus, studying their genetic variation in endangered great ape species is critical. We estimated the sizes (copy number) of nine Y ampliconic gene families in population samples of chimpanzee, bonobo, and orangutan with droplet digital PCR, combined these estimates with published data for human and gorilla, and produced genome-wide testis gene expression data for great