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The effective control of the release of endogenous phosphorus is an urgent problem in the management of urban malodorous rivers. This research explored the fraction and regeneration of phosphorus of urban malodorous river in the context of sulfate reduction. It was found that sulfate reduction could promote sediment phosphorus release. The contents of total phosphorus (TP) and soluble reactive phosphorus (SRP) in the overlying water presented a decreasing trend after the initial increase during the operation of 120 days. The phosphorus