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in a wider range of pH values (3.0-8. in comparison to the wild type enzyme. In contrast to the natural laccase, these mutants efficiently degraded bulky and harmful triphenylmethane dyes such as Ethyl Green (up to 91.64% after 24 h), even in the absence of mediators, with positive implications for the use of such modified laccases in many green chemistry processes (e.g. wastewater treatment).The history of modern biochemistry started with the cellular theory of life. By putting aside the holistic protoplasmic theory, scientists o