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2D and 3D ALG scaffolds were ready and characterized in terms of water content, swelling, technical weight and morphology before real human fibroblast seeding (8days). Using a liquid chromatography-triple quadrupole-tandem mass spectrometry approach, chosen ceramides (CER), lysophosphatidylcholines (LPC), lysophosphatidic acids (LPA) and free efas (FFA) were reviewed. The results revealed a definite alteration within the CER expression profile based of both the geometry