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Dyes are an important class of organic pollutants and are well known for their adverse effects on aquatic life and human beings. In this work, an effort has been made to treat the dye-containing wastewater using modified biocarriers in packed bed bioreactors (PBBRs). Lysinibacillus sp. immobilized polyurethane foam combined with activated carbon and sodium alginate was used for the biodegradation of Congo red dye. The optimum values of process time, glucose concentration, and dye concentration were obtained to be 4.0 days, 2.0 g/L, and