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Furthermore, we identified the ketoacid decarboxylase from Carnobacterium maltaromaticum become the right alternative for isobutanol manufacturing, since replacement of kivD from L. lactis in P. putida Iso2 because of the variant from C. maltaromaticum yielded an identical YIso/S. Although P. putida is regarded as obligate cardiovascular, we show that under oxygen starvation conditions this bacterium does not develop, remains metabolically energetic, and therefore engineer