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As one example, Pseudomonas aeruginosa biofilm is grown from single cells, which is shown that (1) the platform delivers reproducible and trustworthy outcomes; (2) development is based on flow rate and development medium composition; and (3) efficacy of antibiotic drug treatment depends on the shaped biofilm. This system allows biofilm growth, quantification, and therapy such as a conventional movement setup while lowering the applying barri
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