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The objective of this study is to apply pubertal stage estimation methods to a sample from a rural community the post-medieval Dutch skeletal collection from Middenbeemster. Puberty is a key developmental period involving transition to physical adulthood with broad societal relevance through its impact on fertility, morbidity, and mortality. Individuals (n = 55), including 27 of known sex and age-at-death, between the ages of 8 and 25 years were assessed for six skeletal markers indicative of pubertal growth spurt. Recent novel osteoar