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6%), respectively. Overall, 100% mortality was observed on the 8th and 9th days in treated mice with the concentrations of 0.2 and 0.4 mL/kg, respectively. The mean number of tachyzoites in the mice treated with 0.2 and 0.4 mL/kg of ZM-EO were 189×104 and 76×104 cell/mL, respectively, meaningfully (P less then 0.05) reduced compared with the control mice. Results also demonstrated that ZM-EO had no important toxicity on mice. Conclusion The results demonstrated the efficacy of ZM-EO against acute toxoplasmosis. Nevertheless, supplemen