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This analysis will discuss about a development of a Flow Injection Analysis-Atomic Absorption Spectrometry (FIA-AAS) method that uses bentonite based useful material as a retention column. The split, preconcentration and trace analysis of the Cr types in the water is successfully performed using a Bt-MCCs mini-column within the FIA-AAS system. Analytical performance for the evolved method is referred to as repeatability, linearity, and recognition limitation. Analytical