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Microplastic pollution is a major global challenge and a severe threat to aquatic organisms. The Pearl River Basin is the most important river system in Guangdong Province, China. As the second largest tributary of the Pearl River Basin, the Beijiang River flows through many cities in Guangdong Province and eventually merges into the Pearl River Delta. The goal of this research was to explore the microplastic pollution problem in the Beijiang River and Pearl River Delta. In total, 26 surface water and 47 wild freshwater fish samples from