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The production profile of aspirin through the prepared membrane layer was also measured by ultraviolet spectrophotometry. Additionally, the antibacterial activities associated with membrane layer ended up being assessed. We also assessed the inside vitro results of the prepared membrane in the biocompatibility and osteogenic differentiation of dental care pulp stem cells (DPSCs), and evaluated in vivo bone tissue regeneration utilizing the prepared membrane within the flaws created in both sides regardi