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Increased positive selection of SARS-CoV-2 were statistically significant during the quarantine (Tajima's -2.03, 0.01; Fu and Li's -2.66, 0.01; and Zeng's -2.37, 0.01). Linkage disequilibrium analysis confirmed that ribonucleic acid (RNA) recombination with the11083G T mutation also contributed to the increase of mutations among the viral progeny. The findings indicate that the 11083G T mutation of SARS-CoV-2 spread during shipboard quarantine and arose through RNA recombination under positive selection press