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To increase xylose concentration of the chestnut shell hemicellulosic hydrolysate with an acceptable phenolic compound level in order to enhance xylitol production by Candida tropicalis M43. The xylose concentration and total phenolic compound concentration of the hydrolysate were obtained as 33.68g/L and 77.38mg gallic acid equivalent/L, respectively by optimization of detoxification parameters and concentration level (60°C, 115min contact time, 5.942% (w/v) dosage of activated charcoal, 120 strokes/min shaking rate and 0.2 volume ratio