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The constant need of humans and animals for food resources was led to overuse of antibiotics as vital medicines. In this regard, we are now facing major concern about the risks on the food safety and environment owing to their uncontrolled disposal. Hence, the progress of simple and sensitive approaches for fast monitoring of antibiotic levels is highly desirable. Here, we aimed to describe a new sensitive and easy-to use strategy based on electrochemical single off apta-assay toward ciprofloxacin (CFX). A novel interface using 3D Au-PA