https://www.selleckchem.com/pr....oducts/syrosingopine
Visualizing the spatiotemporal organization of the genome will improve our understanding of how chromatin structure and function are intertwined. Here, we describe the further development of the RNA-guided endonuclease-in situ labeling (RGEN-ISL) method CRISPR-FISH. Using soybean and mouse chromosomes, we demonstrate that the treatment of conventionally fixed chromosomes (in ethanol or methanolacetic acid) with 40 mM Tris-HCl (pH 9. for 30 minutes at 37°C prior to CRISPR-FISH allows the application of this method for the
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