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The naturally occurring spermine (Spm), as one of the many cationic polyamines, plays a key role in biological processes and is involved in a variety of diseases. The very similar structures among biogenic polyamines present a major challenge to achieve discriminative testing among them. In this contribution, using arbitrary ssDNA-coated Au@PtNPs as the probe, we demonstrated that a dual-mode strategy via a gas pressure and colorimetric signal readout can be used for the sensitive and specific detection of Spm, due to the target-respon