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Haematococcus pluvialis is a commercial microalgae used for natural astaxanthin production. This study aims to investigate the roles of melatonin (MT) and 3-methyladenine (3-MA) in regulating the cell growth and biosynthesis of astaxanthin and fatty acids under adverse conditions by H. pluvialis. Upon the dual treatments, the maximum astaxanthin concentration (46.78 mg L-1) was 2.39- and 1.35-fold higher compared with the control and MT treatments, respectively. Concomitantly, the combined application of MT and 3-MA suppressed autophagy
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