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The antibiotic distributions, partitioning, and migration pathways in river basins have withdrawn great attention in the past decades. This study investigates 26 antibiotics of five classifications in surface water and sediment samples at 23 sites in Fenhe River, a typical tributary of Yellow River. There are 21 antibiotics found in the water samples at the concentration from 113.8 to 1106.0 ng/L, in the decreasing order of SAs QNs MLs TCs CAs. Fifteen antibiotics were detected in the sediment at the concentrations from