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In silico tools for the prediction of disintegration and/or dissolution of tablets can be validated using adequate images of real pharmaceutical formulations. X-ray micro-computed tomography images of 12 different tablet batches prepared from binary mixtures of API and excipient were used. The goal of this work was to compare different segmentation methods to improve the results and processing time of an evaluation of pre-processing methods. The open source software ImageJ was utilised for the image processing. Different threshold algor