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The results show that the species-specific hydrolysis probe assay was successfully developed for the raw materials and powders of A. racemosa. The specificity of the test was 100% to the target species. The efficiency of the assay was observed to be 99%, and the reliability of the assay was 100% for the raw/starting and powder materials. The method developed in this study can be used to authenticate and perform qualitative analysis of A. racemosa supplements. The method developed in this study can be used to authenticat