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Collection through the endoscope suction channel was more advanced than collection with a catheter. Collection beyond 8 minutes reduced biomarker yield. PJ-derived organoid tradition was possible. The optimal protocol for secretin-stimulated PJ collection is through the endoscope suction channel for 8 minutes allowing biomarker detection and organoid culture.The perfect protocol for secretin-stimulated PJ collection is by the endoscope suction channel for 8 minutes allowing biomarker detection and organoid tra