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This property is pertinent since the design of sophisticated circuits is no longer required to fully homogenize the droplet content before characterization, opening broader perspectives in droplet-based microfluidics.Rapid and sensitive detection of Hg2+ in the environment and drinking water is vital because of its non-degradability, bioaccumulation, and high toxicity. Herein, we report a portable evanescent wave optofluidic biosensor (EWO for simple sensitive detection of Hg2+ using fluorescence labeled poly-A DNA strand (CY-A14) an