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This AgNCs-based light up strategy provides a potential tool for further investigation of protein dimerization on cell surface, which is more conducive to the mechanism research, accurate classification and treatment of cancer.Labile Zn(II) species play key roles in inducing bioresponse. Thus the development of a biosensor for labile Zn(II) quantification is important. In this study, we demonstrate that the autofluorescence intensity (FITC channel) of an adenine deficient yeast (Ade(-) yeast) was enhanced in the presence of Zn2+. Yeast