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70-fold increase in the permeability coefficient across porcine buccal tissue. These findings were further corroborated based on bioimaging analysis using CLSM. SGDC-ELs showed the greatest fluorescence intensity in buccal tissues, as evidenced by the greater shift of fluorescence intensity toward the inner buccal tissue over time. The fluorescence intensity ranked as follows SGDC-EL SC-EL FITC-insulin solution. Conclusively, this study highlighted the potential nanocarriers for enhancing the buccal permeability of insulin.Ra