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Horses lose high amounts of Na through excessive sweating. These fluid losses can often not be replaced completely by voluntary water intake, requiring saline solutions as rehydration therapy to regain electrolyte balance. The experiment aimed to evaluate the sensitivity and tolerance of Shetland ponies towards different Na concentrations in their drinking water and contained three phases (1) control only fresh water provided; (2) pairwise-preference test choice between fresh water and saline solution with stepwise increasing sodium chlor