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CONCLUSIONS We established a mass spectrometry-based immunoassay for the simultaneous detection of PGI and PGII in a single analysis. The element tagged immunoassay coupled with ICP-MS detection has high sensitivity, accuracy, and specificity in clinical serum sample analysis. © 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc.BACKGROUND Blood gas analyzers are capable of delivering results on electrolytes and metabolites within a few minutes and facilitate clinical decision-making. However,