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Surface electromyography (sEMG) is an easy, noninvasive, and reproducible way to assess spontaneous electrical activity of muscles in real time. In this study, we report data on the correlation between sEMG and mimetic muscle activity during specific tasks so as to create a case-control reference for future studies on acute, chronic, and congenital facial palsy. Twelve healthy participants were enrolled (6 women and 6 men) with a mean age of 42.75 (range, 26-58 years) years. sEMG signals were recorded at rest and while performing volunt