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Petrol chromatography-mass spectrometry (GC-MS) exposed this fraction to an acetylation a reaction to determine the information associated with the additional metabolites. The GC-MS evaluation showed that the F5 fraction had been consists of trehalose CAS 6138-23-4, polyols (L-iditol CAS 488-45-9, galactitol CAS 608-66-2, D-mannitol CAS 69-65-8, D-glucitol CAS 50-70-4, and myoinositol CAS 87-89-8), adipic acid CAS 124-04-9, stearic acid CAS 57-11-4, squalene CAS 111-02-4, and β-sitosterol CAS 83-46-5.Good high qua