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In the current study, a soil bacterial isolate F2 expressed a significant antagonistic activity against Candida albicans ATCC 10231 and Aspergillus niger clinical isolate confirmed through cross streak, dual culture, and agar well diffusion methods. The isolate F2 was identified using phenotypic and molecular approaches as Alcaligenes (A.) faecalis MT332429. The identification and structural characterization of the antifungal compound was performed using advanced spectroscopic techniques including UV absorbance, 1H and 13C NMR and 2D N