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In a bleomycin-induced mouse pulmonary fibrosis design, msFGFR2c alleviate pulmonary fibrosis and suppress the reduction in pro-SPC amounts. Thus, msFGFR2c can restrict EMT-induced fibrosis of AE2 cells via FGF-2 sign and AE2 cells is suggested to relax and play a crucial role within the lung fibrotic process.OBJECTIVE to build up a convenient double-locus scarless genome editing system in Escherichia coli, on the basis of the kind II Streptococcus pyogenes CRISPR/Cas9 and λ Red r