https://www.selleckchem.com/pr....oducts/Monensin-sodi
Intestinal microbiota and metabolites play important roles for further improvement of animal production. Metabolomics of shrimp intestine to understand roles and their relationship to the host is hampered by the lack of metabolome profiling method. This study aims to develop extraction and analytical methods to allow accurate metabolic analysis in shrimp intestine. Conditions for extraction and LC-HRMS/MS analysis were optimized. Extraction with ethyl acetateacetone (152 v/v) acidified with 0.5% acetic acid, elution