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Nef also promoted autophagy induction through increasing beclin-1, atg-4, atg-5 and atg-12, LC-3 activation, and P 62/SQSTM1 as determined by western blot analysis. Collectively, these results demonstrate that Nef is a potent anticancer compound against cervical cancer cells through inducing apoptosis and autophagic pathway involving ROS. © 2020 John Wiley Sons, Ltd.Axially chiral indole-based frameworks have been recognized as a class of important five-membered (hetero)biaryls, and developing catalytic asymmetric approaches for c