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These results suggest that 4years of SLIT is needed to achieve sustained increases in Foxp3 Treg cells, which are closely associated with the efficacy of SLIT. These results suggest that 4 years of SLIT is needed to achieve sustained increases in Foxp3+ Treg cells, which are closely associated with the efficacy of SLIT.The workshop “Application of evidence-based methods to construct mechanistic frameworks for the development and use of non-animal toxicity tests” was organized by the Evidence-based Toxicology Collaboration and hosted by