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Recently, it was stated that some unsaturated lipids associated with pulmonary surfactants are oxidized by low-level ozone in background environment. Nevertheless, the molecular-level comprehension of the effect mechanism is still limited due to technical problems. We applied heterodyne-detected amount frequency generation (HD-SFG) spectroscopy to probe the effect procedure for an unsaturated phospholipid monolayer (1-palmitoyl-2-oleoyl-glycero-3-phosphocholine, POPC), which can be among the
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